Zainuddin Basri
Faculty Of Agriculture, Tadulako University, Indonesia, Indonesia

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Journal : AGROLAND: The Agricultural Sciences Journal

THE STRENGTH OF MS MEDIA AND STERILIZATION TECHNIQUE ON RED DRAGONFRUIT (HYLOCEREUS POLYRHIZUS) SEED GERMINATION Hawalina Kasim; Yusran Yusran; Zainuddin Basri
AGROLAND: The Agricultural Sciences Journal Vol 2, No 1 (2015)
Publisher : Tadulako University

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Abstract

This research aimed to findout efficient sterilization method and MS media to germinate dragonfruit seeds in vitro, conducting at Biotechnology Laboratory, Agriculture Faculty, Tadulako University, using factorial completely randomized design. The first factor was sterilization technique (S), i.e: S1 (The sliced fruit was washed with sterile distilled water three times, then rinsed in Bayclin (household bleach containing 5.25% NaOCl) 5% for 15 minutes, followed by washing in sterile distilled water three times. S2 (The seeds were taken from the fruit meat, then rinsed in 5% Bayclin for 15 minutes, then washed with sterile distilled water three times. S3 (The seeds were taken from the fruit meat, then rinsed in 15% Bayclin for 15 minutes, then washed with sterile distilled water three times. The second factor was the strength of MS media (M), i.e full strength MS (M1) and half strength MS for macro and micro nutrients (M2). Each treatment combination consisted of 35 seeds, and was replicated three times. Germination responses were observed as time to germinate, germination percentage and percentage of opened cotyledone seedlings. All datas were subjected to Analysis of Variance and the mean differences among the treatments were analyzed using Honest Significant Difference (HSD) at the level of 1%. The results showed that removing seed pulp, prior to rinsing the seeds in 15% Bayclin for 15 minutes followed by washing in sterile aquadest three times, and cultured in half MS produce the fastest and highest seed germination of 99.05% with 92.38% opened cotyledon seedlings after 2 weeks in culture.   Key Words: Dragonfruit, germination, in vitro, MS strength, sterilization.
INDICATOR DETERMINATION OF SELECTION FOR IMPROVING SOYBEAN YIELD Sakka Samudin; Alfian Samudin; Zainuddin Basri
AGROLAND: The Agricultural Sciences Journal Vol 1, No 1 (2014)
Publisher : Tadulako University

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Abstract

The need for soybean increasing every year causes soybean imports to be done continually, so an effort to increase the production must be done to reduce dependency on imported soybean. This study aims to determine the characters that can be used as an indicator of selection to increase soybean yields. The study was conducted in the village of Sa'atu, Coastal Poso Subdistrict, Poso Regency, Central Celebes Province, from January to April 2011. Using a randomized block design (RBD) with 30 genotypes (varieties) as the treatments that were repeated 3 times, so it obtained 90 units of experimental plots. The observed variables were plant height at harvesting time, flowering age, harvesting age, number of pods per plant, number of seeds per pod, number of productive branches per plant, total weight of pods per plot, weight of 100 seeds and yield of seeds. The results showed that plant height at harvesting time, harvesting age and number of seeds per pod have genetic variability coefficients that were moderate while the other traits were high. The number of pods per clump and the number of seeds per pod have moderate heritability values while the other traits were high enough. Harvesting age has a significant negative genetic correlation with the yield while the number of productive branches, total weight of pods per plot and weight of 100 seeds have significant positive genetic correlations with soybean yields. Weight of pods per plot is a trait that has high direct effect on and significant positive correlation with the yields so it can be used as an indicator of trait selection to improve soybean yields.
CALLUS INDUCTION OF CACAO CLONE SULAWESI 1 ON VARIOUS CONCENTRATIONS OF 2,4 -D AND COCONUT WATER VIA IN VITRO CULTURE Asmila Asmila; Zainuddin Basri; Ramal Yusuf; Hawalina Hawalina
AGROLAND: The Agricultural Sciences Journal Vol 4, No 1 (2017)
Publisher : Tadulako University

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Abstract

Cacao is one of important plantation crops grouped in the Sterculiaceae family.  Sulawesi is the main area of cacao production and has a number of superior clones, such as Sulawesi 1 and Sulawesi 2.  Based on data in 2012/2014 cacao production to consumption reached 174,000 tons, while in 2013/2014 was projected a deficit of 115,000 tonnes. Nonetheless, cacao agribusiness in Indonesia is still facing complex problems, among others gardener productivity is still low due to borer attacks cacao, the quality of products and the number is still low and still not optimal development of cacao products and providing superior amount of cacao seedlings. The primary problem of cacao production recently is low productivity.  The main cause of low cacao productivity in Central Sulawesi is the use of inferior clones.  To enhance cacao productivity, it is crucial to use cacao clones having high genetic potential via tissue culture or micropropagation techniques.  The aim of this experiment was to assess the effect of different concentrations of 2,4-D and coconut water on the growth of cacao callus via in vitro culture.  This experiment used Completely Randomozed Block Design in factorial patteren with treatments tested namely 2,4-D and coconut water concentrations.  The concentrations of 2,4-D tested including 1 ppm, 2 ppm and 3 ppm, whilst coconut water concentrations tested consisting of 10%, 15% and 20%, and therefore there were 3 x 3 = 9 treatment combinations.  Each treatment utilized 4 replications; and each unit combination used 5 explants (staminodia).  Results of this experiment indicated that the addition of 3 ppm 2,4-D and 10% coconut water had a highly significant effect on the callus color 4 weeks after culture.  The addition of 3 ppm 2,4-D in culture media showed a significant effect on callus color 4 weeks after culture, but had an insignificant effect on the callus formation, callus color 8 weeks after culture an callus texture.  Supplementation of 20% coconut water had a significant effect on callus texture 8 weeks after culture, whilst the addition of 10% coconut water showed a significant effect on callus color 4 weeks after culture.